Hotspot

psbM-trnD-GUC

LSC · 30206-31043 · 838 bp

Back to Alstonia

Pi 0.073

MarkerSeek score 86.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 838
variable_sites: 85
pi: 0.073
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 86.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AAATGCAAGAATATTTACTTCCA	TTCAATTGGTCAGAGCACCG	26.1	50.0	52.7	58.5	926-946 bp	1.0	51.3
psbM-trnD-GUC_p2	AATGCAAGAATATTTACTTCCAT	TTCAATTGGTCAGAGCACCG	26.1	50.0	52.4	58.5	925-945 bp	1.0	51.3
psbM-trnD-GUC_p3	AAATGCAAGAATATTTACTTCCAT	TTCAATTGGTCAGAGCACCG	25.0	50.0	53.3	58.5	926-946 bp	1.0	51.3
psbM-trnD-GUC_p4	AAATGCAAGAATATTTACTTCCA	GTTCAATTGGTCAGAGCACC	26.1	50.0	52.7	57.0	927-947 bp	1.0	51.3
psbM-trnD-GUC_p5	AATGCAAGAATATTTACTTCCAT	GTTCAATTGGTCAGAGCACC	26.1	50.0	52.4	57.0	926-946 bp	1.0	51.3