Hotspot

petN-psbM

LSC · 29039-30100 · 1062 bp

Back to Alstonia

Pi 0.0875

MarkerSeek score 84.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1062
variable_sites: 125
pi: 0.0875
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGGTTGGAGAGCCCGATAG	TTCAAAACCAGCGGGATTCA	55.0	45.0	58.0	58.0	1982 bp	0.333333	53.2
petN-psbM_p2	ACTCGTAGTGTGGGGAAGAA	TTCAAAACCAGCGGGATTCA	50.0	45.0	58.0	58.0	1312 bp	0.333333	53.2
petN-psbM_p3	TCGTAGTGTGGGGAAGAAGT	TTCAAAACCAGCGGGATTCA	50.0	45.0	58.0	58.0	1310 bp	0.333333	53.2
petN-psbM_p4	AGTGTCTAATGGCTGGACCT	TTCAAAACCAGCGGGATTCA	50.0	45.0	58.0	58.0	2007 bp	0.333333	52.9
petN-psbM_p5	GAATTAGGTTGGAGAGCCCG	TTCAAAACCAGCGGGATTCA	55.0	45.0	58.0	58.0	1986 bp	0.333333	52.9