Hotspot

atpH-atpI

LSC · 13450-14501 · 1052 bp

Back to Alstonia

Pi 0.0718

MarkerSeek score 84.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1052
variable_sites: 108
pi: 0.0718
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TGCTTTGGCTTTACTCACGT	45.0	45.0	58.0	58.0	1415-1467 bp	1.0	88.7
atpH-atpI_p2	ATAAACAACCCGCACACACT	TGCTTTGGCTTTACTCACGT	45.0	45.0	58.0	58.0	2122-2168 bp	1.0	88.6
atpH-atpI_p3	ATAAACAACCCGCACACACT	GAATACGGTCCTTGGGTTCC	45.0	55.0	58.0	58.0	2268-2314 bp	1.0	88.2
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	GAATACGGTCCTTGGGTTCC	45.0	55.0	58.0	58.0	1561-1613 bp	1.0	88.1
atpH-atpI_p5	ATAAACAACCCGCACACACT	TTCGGAACCCACAAACCATT	45.0	45.0	58.0	57.9	2363-2409 bp	1.0	87.6