Hotspot

trnS-GCU-trnG-UCC

LSC · 8423-8943 · 521 bp

Back to Alstonia

Pi 0.0912

MarkerSeek score 83.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 521
variable_sites: 63
pi: 0.0912
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	566-601 bp	1.0	63.4
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	45.0	36.4	58.0	56.2	567-602 bp	1.0	61.5
trnS-GCU-trnG-UCC_p3	GCTTTAGTCCACTCAGCCAT	ACGAATCACACTTTTACCACT	50.0	38.1	57.6	55.5	552-587 bp	1.0	60.4
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	TACCACTAAACTATACCCGC	45.0	45.0	58.0	54.0	552-587 bp	1.0	59.6
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	ACGAATCACACTTTTACCACT	55.0	38.1	58.5	55.5	547-582 bp	1.0	59.6