Hotspot

trnR-ACG-trnT-UGU

Genome · 153395-154860 · 1466 bp

Back to Alsophila

Pi 0.03

MarkerSeek score 54.10

Primer available yes

Label trnR-ACG-trnT-UGU

Feature Metadata

label_name: trnR-ACG-trnT-UGU
feature_type: igs
region: Genome
length_bp: 1466
variable_sites: 68
pi: 0.03
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnT-UGU_p1	AAAAGTGGTTCCTGGAACGG	GTTCGAATCCTCCGCGAG	50.0	61.1	58.3	58.0	1059-1595 bp	1.0	69.6
trnR-ACG-trnT-UGU_p2	AAGTGGTTCCTGGAACGGAT	GTTCGAATCCTCCGCGAG	50.0	61.1	58.9	58.0	1057-1593 bp	1.0	64.6
trnR-ACG-trnT-UGU_p3	AAAAGTGGTTCCTGGAACGG	ACGGGGGTTCGAATCCTC	50.0	61.1	58.3	58.7	1065-1601 bp	1.0	64.1
trnR-ACG-trnT-UGU_p4	AAAAGTGGTTCCTGGAACGG	GACGGGGGTTCGAATCCT	50.0	61.1	58.3	58.7	1066-1602 bp	1.0	64.1
trnR-ACG-trnT-UGU_p5	AAAAGTGGTTCCTGGAACGG	AGGACGGGGGTTCGAATC	50.0	61.1	58.3	58.7	1068-1604 bp	1.0	64.0