Hotspot

rps4-trnL-CAA

Genome · 47368-48029 · 662 bp

Back to Alsophila

Pi 0.0444

MarkerSeek score 60.20

Primer available yes

Label rps4-trnL-CAA

Feature Metadata

label_name: rps4-trnL-CAA
feature_type: igs
region: Genome
length_bp: 662
variable_sites: 51
pi: 0.0444
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-CAA_p1	GAGGTCCTCGGTAGCGAG	CTGAATCTGGGAGCTGTCAC	66.7	55.0	58.6	58.0	772-804 bp	1.0	64.4
rps4-trnL-CAA_p2	GAGGTCCTCGGTAGCGAG	TAGGTTCCCCTGAATCTGGG	66.7	55.0	58.6	58.1	781-813 bp	1.0	63.7
rps4-trnL-CAA_p3	GAGGTCCTCGGTAGCGAG	AATCTGATTGAGTCCGCAGC	66.7	50.0	58.6	58.3	727-759 bp	1.0	62.2
rps4-trnL-CAA_p4	GAGGTCCTCGGTAGCGAG	GAATCTGGGAGCTGTCACTC	66.7	55.0	58.6	57.7	770-802 bp	1.0	62.1
rps4-trnL-CAA_p5	GGTCCTCGGTAGCGAGAC	CTGAATCTGGGAGCTGTCAC	66.7	55.0	58.9	58.0	770-802 bp	1.0	61.9