Hotspot

ycf3-trnS-GGA

Genome · 45705-46202 · 498 bp

Back to Alsophila

Pi 0.0461
MarkerSeek score 73.80
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
Genome
length_bp
498
variable_sites
39
pi
0.0461
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 AGTGGTCGGTAGAATTTGTAACA TGCTACACCTTAAACCGCTC 39.1 50.0 57.8 57.9 599-617 bp 1.0 61.8
ycf3-trnS-GGA_p2 AGTGGTCGGTAGAATTTGTAACA GTGCTACACCTTAAACCGCT 39.1 50.0 57.8 58.2 600-618 bp 1.0 61.0
ycf3-trnS-GGA_p3 GTCATTTCTCTGAGATCTAGGCA TGCTACACCTTAAACCGCTC 43.5 50.0 57.5 57.9 536-554 bp 1.0 60.3
ycf3-trnS-GGA_p4 AGTGGTCGGTAGAATTTGTAACA CTACACCTTAAACCGCTCGG 39.1 55.0 57.8 58.4 597-615 bp 1.0 59.7
ycf3-trnS-GGA_p5 TCTCTTTTAGTGGTCGGTAGA TGCTACACCTTAAACCGCTC 42.9 50.0 55.5 57.9 607-625 bp 1.0 59.3