Hotspot

psbD-trnT-GGU

Genome · 36201-37828 · 1628 bp

Back to Alsophila

Pi 0.0384

MarkerSeek score 72.30

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1628
variable_sites: 106
pi: 0.0384
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	ACATAAAAGGGCAGCACCAA	CTTGAGGTCACGGGTTCAAA	45.0	50.0	58.0	58.0	2719-2790 bp	1.0	86.6
psbD-trnT-GGU_p2	ACATAAAAGGGCAGCACCAA	ATAGGCCACCCTTCCCTATC	45.0	55.0	58.0	58.0	2653-2724 bp	1.0	86.6
psbD-trnT-GGU_p3	ACATAAAAGGGCAGCACCAA	GATAGGCCACCCTTCCCTAT	45.0	55.0	58.0	58.0	2654-2725 bp	1.0	86.6
psbD-trnT-GGU_p4	ACATAAAAGGGCAGCACCAA	ATGCCACGTAGAATTTCCCC	45.0	50.0	58.0	57.9	2371-2379 bp	0.6	64.4
psbD-trnT-GGU_p5	ACATAAAAGGGCAGCACCAA	TTCCCTATCGAGCTTACCGT	45.0	50.0	58.0	57.9	2703-2713 bp	0.6	64.4