Hotspot

trnS-GCU-psaM

Genome · 7972-8302 · 331 bp

Back to Alsophila

Pi 0.0338

MarkerSeek score 67.80

Primer available yes

Label trnS-GCU-psaM

Feature Metadata

label_name: trnS-GCU-psaM
feature_type: igs
region: Genome
length_bp: 331
variable_sites: 19
pi: 0.0338
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psaM_p1	ATTAGCAATCCGACGCGTTA	GTTGCGAGATTGGCTATTGC	45.0	50.0	57.8	57.9	399-423 bp	1.0	82.9
trnS-GCU-psaM_p2	TTAGACCTCTCGGCCATCTC	GTTGCGAGATTGGCTATTGC	55.0	50.0	58.3	57.9	382-406 bp	1.0	82.4
trnS-GCU-psaM_p3	ATTAGCAATCCGACGCGTTA	TTGCGAGATTGGCTATTGCA	45.0	45.0	57.8	58.3	398-422 bp	1.0	81.8
trnS-GCU-psaM_p4	ATTAGCAATCCGACGCGTTA	GGCTATTGCACTGTACCGTT	45.0	50.0	57.8	58.3	388-412 bp	1.0	81.8
trnS-GCU-psaM_p5	ATTAGCAATCCGACGCGTTA	TTGGCTATTGCACTGTACCG	45.0	50.0	57.8	58.3	390-414 bp	1.0	81.8