Hotspot

matK-rps16

Genome · 3034-3950 · 917 bp

Back to Alsophila

Pi 0.0426

MarkerSeek score 66.90

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: Genome
length_bp: 917
variable_sites: 68
pi: 0.0426
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	GAATCTGCAGTCCAGCTACC	GGGCAAAGGTACCTGAACAA	55.0	50.0	58.1	58.0	1229-1457 bp	1.0	85.0
matK-rps16_p2	GAATCTGCAGTCCAGCTACC	AGAGGATACCCGATACGCAA	55.0	50.0	58.1	58.0	1705-1718 bp	0.6	64.8
matK-rps16_p3	TCCGAGACAGAAGGGGTATC	AGAGGATACCCGATACGCAA	55.0	50.0	57.9	58.0	1779-1780 bp	0.4	55.4
matK-rps16_p4	TCCGAGACAGAAGGGGTATC	GGGCAAAGGTACCTGAACAA	55.0	50.0	57.9	58.0	1301-1302 bp	0.4	55.4
matK-rps16_p5	CCGAGACAGAAGGGGTATCT	AGAGGATACCCGATACGCAA	55.0	50.0	57.9	58.0	1778-1779 bp	0.4	55.3