Hotspot

ycf2

Genome · 90068-96925 · 6858 bp

Back to Alseodaphnopsis

Pi 0.00225

MarkerSeek score 54.70

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 6858
variable_sites: 40
pi: 0.00225
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CGACATTGCACGATCAAAGG	GCGAACCCCAGTTAGATTCA	50.0	50.0	57.8	57.6	316 bp	1.0	76.8
ycf2_p2	CGACATTGCACGATCAAAGG	GATCACATTCCTCCATCGCC	50.0	55.0	57.8	58.5	336 bp	1.0	76.0
ycf2_p3	CGACATTGCACGATCAAAGG	ACGATCACATTCCTCCATCG	50.0	50.0	57.8	57.5	338 bp	1.0	75.7
ycf2_p4	CGACATTGCACGATCAAAGG	CGCGAACCCCAGTTAGATTC	50.0	55.0	57.8	58.7	317 bp	1.0	74.3
ycf2_p5	CGACATTGCACGATCAAAGG	CGATCACATTCCTCCATCGC	50.0	55.0	57.8	58.9	337 bp	1.0	73.0