Hotspot

ycf1

Genome · 128511-134075 · 5565 bp

Back to Alseodaphnopsis

Pi 0.00519

MarkerSeek score 44.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5565
variable_sites: 65
pi: 0.00519
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTATTCTGATCCGGATCCGC	CGACCGAAAAACTATCCCCC	55.0	55.0	58.0	58.3	781-799 bp	1.0	80.9
ycf1_p2	GTATTCTGATCCGGATCCGC	GACCGAAAAACTATCCCCCG	55.0	55.0	58.0	58.3	780-798 bp	1.0	80.9
ycf1_p3	TTGCCTCTTCCTCCTCAGAA	CGACCGAAAAACTATCCCCC	50.0	55.0	58.0	58.3	1083-1101 bp	1.0	80.1
ycf1_p4	TTGCCTCTTCCTCCTCAGAA	GACCGAAAAACTATCCCCCG	50.0	55.0	58.0	58.3	1082-1100 bp	1.0	80.1
ycf1_p5	TTTGCCTCTTCCTCCTCAGA	GACCGAAAAACTATCCCCCG	50.0	55.0	58.0	58.3	1083-1101 bp	1.0	80.1