Hotspot

ccsA-ndhD

Genome · 119941-120174 · 234 bp

Back to Alseodaphnopsis

Pi 0.0157

MarkerSeek score 55.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: Genome
length_bp: 234
variable_sites: 7
pi: 0.0157
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGATATGCTATTTTGGGGTCA	TCCTGATTTCGTTCTCTCGC	40.9	50.0	57.2	57.7	369-387 bp	1.0	59.5
ccsA-ndhD_p2	TGGATATGCTATTTTGGGGTCA	CCTGATTTCGTTCTCTCGCT	40.9	50.0	57.2	57.7	368-386 bp	1.0	59.5
ccsA-ndhD_p3	TGGATATGCTATTTTGGGGTCAA	TCCTGATTTCGTTCTCTCGC	39.1	50.0	57.9	57.7	369-387 bp	1.0	56.9
ccsA-ndhD_p4	TTGGATATGCTATTTTGGGGTCA	TCCTGATTTCGTTCTCTCGC	39.1	50.0	57.9	57.7	370-388 bp	1.0	56.9
ccsA-ndhD_p5	TGGATATGCTATTTTGGGGTCAA	CCTGATTTCGTTCTCTCGCT	39.1	50.0	57.9	57.7	368-386 bp	1.0	56.8