Hotspot

ycf3-trnS-GGA

Genome · 46718-47530 · 813 bp

Back to Alseodaphnopsis

Pi 0.00553

MarkerSeek score 59.40

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: Genome
length_bp: 813
variable_sites: 11
pi: 0.00553
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TCTCCTGAGGTTGTCGGAAT	TTGTGGTCGGTTGGTGAATT	50.0	45.0	58.1	57.9	1023-1035 bp	1.0	80.3
ycf3-trnS-GGA_p2	TCTCCTGAGGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.1	58.2	993-1005 bp	1.0	79.6
ycf3-trnS-GGA_p3	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.1	57.8	943-955 bp	1.0	79.3
ycf3-trnS-GGA_p4	TCTCCTGAGGTTGTCGGAAT	ATTGTGGTCGGTTGGTGAAT	50.0	45.0	58.1	57.7	1024-1036 bp	1.0	78.5
ycf3-trnS-GGA_p5	TTCTCCTGAGGTTGTCGGAA	TTGTGGTCGGTTGGTGAATT	50.0	45.0	58.3	57.9	1024-1036 bp	1.0	78.5