Hotspot

trnE-UUC-trnT-GGU

Genome · 32965-33688 · 724 bp

Back to Alseodaphnopsis

Pi 0.00433

MarkerSeek score 54.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: Genome
length_bp: 724
variable_sites: 8
pi: 0.00433
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	824-830 bp	1.0	78.9
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	803-809 bp	1.0	78.8
trnE-UUC-trnT-GGU_p3	CAACCCCCTTTCGTCTTACC	GAACCGATGACTTACGCCTT	55.0	50.0	58.2	58.0	866-871 bp	0.666667	66.0
trnE-UUC-trnT-GGU_p4	CAACCCCCTTTCGTCTTACC	CCATGGCGTTACTCTACCAC	55.0	55.0	58.2	58.1	845-850 bp	0.666667	65.5
trnE-UUC-trnT-GGU_p5	CAACCCCCTTTCGTCTTACC	TGGCGTTACTCTACCACTGA	55.0	50.0	58.2	58.1	842-847 bp	0.666667	65.4