Hotspot

psbM-trnD-GUC

Genome · 31394-32365 · 972 bp

Back to Alseodaphnopsis

Pi 0.0061

MarkerSeek score 62.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: Genome
length_bp: 972
variable_sites: 11
pi: 0.0061
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAACGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	42.9	50.0	58.2	57.9	1084-1140 bp	1.0	70.4
psbM-trnD-GUC_p2	GAATGAACAACGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	56.7	57.9	1083-1139 bp	1.0	70.3
psbM-trnD-GUC_p3	AGAATGAACAACGCAGTAGCA	ATGCTGTACACCCCATTTCC	42.9	50.0	58.2	57.9	1113-1169 bp	1.0	69.9
psbM-trnD-GUC_p4	GAATGAACAACGCAGTAGCA	ATGCTGTACACCCCATTTCC	45.0	50.0	56.7	57.9	1112-1168 bp	1.0	69.8
psbM-trnD-GUC_p5	GAATGAACAACGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	42.9	50.0	57.5	57.9	1083-1139 bp	1.0	68.0