Hotspot

rpoC2-rpoC1

Genome · 21720-21880 · 161 bp

Back to Alseodaphnopsis

Pi 0.0613

MarkerSeek score 72.70

Primer available yes

Label rpoC2-rpoC1

Feature Metadata

label_name: rpoC2-rpoC1
feature_type: igs
region: Genome
length_bp: 161
variable_sites: 18
pi: 0.0613
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rpoC1_p1	GGTGTGCGATGTATATGCCA	AGGCCTACTCATACGGTACC	50.0	55.0	58.1	58.0	298 bp	1.0	85.7
rpoC2-rpoC1_p2	GGTGTGCGATGTATATGCCA	GGCCTACTCATACGGTACCT	50.0	55.0	58.1	58.0	297 bp	1.0	85.7
rpoC2-rpoC1_p3	AGGGTGTGCGATGTATATGC	GGCCTACTCATACGGTACCT	50.0	55.0	57.8	58.0	299 bp	1.0	85.3
rpoC2-rpoC1_p4	AGGGTGTGCGATGTATATGC	AGGCCTACTCATACGGTACC	50.0	55.0	57.8	58.0	300 bp	1.0	85.2
rpoC2-rpoC1_p5	GGTGTGCGATGTATATGCCA	AGAAGAAGCCATACAGGGGT	50.0	50.0	58.1	58.0	324 bp	1.0	85.0