Hotspot

rps16-trnQ-UUG

Genome · 6301-8214 · 1914 bp

Back to Alseodaphnopsis

Pi 0.0037

MarkerSeek score 64.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: Genome
length_bp: 1914
variable_sites: 17
pi: 0.0037
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1850-1987 bp	1.0	83.0
rps16-trnQ-UUG_p2	GGGTACAAGAATCCACAGCC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.2	58.1	1883-2020 bp	1.0	82.1
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1854-1991 bp	1.0	81.9
rps16-trnQ-UUG_p4	CCACAGCCGATAATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	1871-2008 bp	1.0	81.4
rps16-trnQ-UUG_p5	GTACAAGAATCCACAGCCGA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	1881-2018 bp	1.0	81.0