Hotspot

petB

LSC · 78677-80113 · 1437 bp

Back to Alseodaphne

Pi 0.00186

MarkerSeek score 60.00

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1437
variable_sites: 4
pi: 0.00186
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TTTGACCGTGGAATTCCGTT	ACACTATTGTTCCTCCCCGA	45.0	50.0	58.0	58.1	1537-1538 bp	1.0	79.6
petB_p2	CGTGGAATTCCGTTGTTTCG	ACACTATTGTTCCTCCCCGA	50.0	50.0	58.1	58.1	1531-1532 bp	1.0	79.4
petB_p3	TCTGGTAGTTTGACCGTGGA	ACACTATTGTTCCTCCCCGA	50.0	50.0	58.3	58.1	1545-1546 bp	1.0	77.5
petB_p4	ACCGTGGAATTCCGTTGTTT	ACACTATTGTTCCTCCCCGA	45.0	50.0	58.3	58.1	1533-1534 bp	1.0	77.3
petB_p5	CCGTGGAATTCCGTTGTTTC	ACACTATTGTTCCTCCCCGA	50.0	50.0	57.7	58.1	1532-1533 bp	1.0	77.2