Hotspot

ndhF

SSC · 114160-116388 · 2229 bp

Back to Alseodaphne

Pi 0.00479

MarkerSeek score 59.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 16
pi: 0.00479
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCACGTTCAATGGTGGATGA	CCGGAAGAAATAAGGGCTGG	45.0	55.0	57.2	58.3	2396 bp	1.0	71.2
ndhF_p2	ACGTTCAATGGTGGATGACT	CCGGAAGAAATAAGGGCTGG	45.0	55.0	57.1	58.3	2394 bp	1.0	71.1
ndhF_p3	CACGTTCAATGGTGGATGAC	CCGGAAGAAATAAGGGCTGG	50.0	55.0	57.1	58.3	2395 bp	1.0	70.8
ndhF_p4	TCACGTTCAATGGTGGATGA	ATAAGGGCTGGTGAATGGGA	45.0	50.0	57.2	58.4	2387 bp	1.0	70.6
ndhF_p5	ACGTTCAATGGTGGATGACT	ATAAGGGCTGGTGAATGGGA	45.0	50.0	57.1	58.4	2385 bp	1.0	70.5