Hotspot

ndhF-rpl32

SSC · 116389-117622 · 1234 bp

Back to Alseodaphne

Pi 0.00702

MarkerSeek score 49.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1234
variable_sites: 13
pi: 0.00702
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAACAAGCAGAAGTCCCAA	TATCCCCGCCTTACTTTGGA	50.0	50.0	58.0	58.1	1938-1943 bp	1.0	80.0
ndhF-rpl32_p2	AGCAGAAGTCCCAACCCTAT	TATCCCCGCCTTACTTTGGA	50.0	50.0	58.0	58.1	1932-1937 bp	1.0	79.9
ndhF-rpl32_p3	AATAGGGAGGCACACAGAGT	TATCCCCGCCTTACTTTGGA	50.0	50.0	58.0	58.1	2547-2552 bp	1.0	79.7
ndhF-rpl32_p4	AAGGGGAAATTGTGCGGATT	TATCCCCGCCTTACTTTGGA	45.0	50.0	58.1	58.1	2587-2592 bp	1.0	79.5
ndhF-rpl32_p5	AGGGGAAATTGTGCGGATTT	TATCCCCGCCTTACTTTGGA	45.0	50.0	58.1	58.1	2586-2591 bp	1.0	79.5