Hotspot

rpl2-rpl23

LSC · 89557-89589 · 33 bp

Back to Alseodaphne

Pi 0.0444

MarkerSeek score 75.90

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: LSC
length_bp: 33
variable_sites: 2
pi: 0.0444
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	TCGCGGATTGGATTTCACTT	CGCTTCAACCGGGTTATTCT	45.0	50.0	57.9	58.3	169-173 bp	1.0	79.8
rpl2-rpl23_p2	TTCGCGGATTGGATTTCACT	CGCTTCAACCGGGTTATTCT	45.0	50.0	57.9	58.3	170-174 bp	1.0	79.8
rpl2-rpl23_p3	CGCGGATTGGATTTCACTTG	CGCTTCAACCGGGTTATTCT	50.0	50.0	57.8	58.3	168-172 bp	1.0	79.3
rpl2-rpl23_p4	TCGCGGATTGGATTTCACTT	ATCATTACGCTTCAACCGGG	45.0	50.0	57.9	58.3	176-180 bp	1.0	79.1
rpl2-rpl23_p5	TTCGCGGATTGGATTTCACT	ATCATTACGCTTCAACCGGG	45.0	50.0	57.9	58.3	177-181 bp	1.0	79.1