Hotspot

rps19-rpl2

LSC · 87972-88050 · 79 bp

Back to Alseodaphne

Pi 0.0307
MarkerSeek score 60.80
Primer available yes
Label rps19-rpl2

Feature Metadata

label_name
rps19-rpl2
feature_type
igs
region
LSC
length_bp
79
variable_sites
3
pi
0.0307
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-rpl2_p1 TAATGGTAGATGCCCGGGAC GTTATCCTGCGCTTGGAAGA 55.0 50.0 58.9 58.0 272-277 bp 1.0 74.9
rps19-rpl2_p2 TAATGGTAGATGCCCGGGAC CACAACCCCTTGGGGTTATC 55.0 55.0 58.9 58.2 286-291 bp 1.0 73.7
rps19-rpl2_p3 TAATGGTAGATGCCCGGGAC GGCCCCGATTGGTAGAAAAA 55.0 50.0 58.9 58.2 310-315 bp 1.0 73.5
rps19-rpl2_p4 GCCCGGGACCAAGTTACTAT GTTATCCTGCGCTTGGAAGA 55.0 50.0 59.2 58.0 261-266 bp 1.0 73.2
rps19-rpl2_p5 TAATGGTAGATGCCCGGGAC TATCCTGCGCTTGGAAGAAG 55.0 50.0 58.9 57.7 270-275 bp 1.0 72.5