Hotspot

psaA-ycf3

LSC · 44149-44787 · 639 bp

Back to Alseodaphne

Pi 0.00313

MarkerSeek score 60.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 3
pi: 0.00313
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TGGTTGAAGATCGCAAGGAG	50.0	50.0	57.9	57.9	748-749 bp	1.0	78.7
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GTTGAAGATCGCAAGGAGGT	50.0	50.0	57.9	57.9	746-747 bp	1.0	78.7
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	GTCCGATCAAGCTGCTGAAT	50.0	50.0	57.9	58.3	821-822 bp	1.0	76.8
psaA-ycf3_p4	TGTTCTTGAGAAATGGCCGG	TGGTTGAAGATCGCAAGGAG	50.0	50.0	58.5	57.9	773-774 bp	1.0	75.9
psaA-ycf3_p5	TGTTCTTGAGAAATGGCCGG	GTTGAAGATCGCAAGGAGGT	50.0	50.0	58.5	57.9	771-772 bp	1.0	75.9