Hotspot

petN-psbM

LSC · 30265-31333 · 1069 bp

Back to Alseodaphne

Pi 0.00187

MarkerSeek score 60.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1069
variable_sites: 3
pi: 0.00187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCGTTGTTCATTCT	50.0	42.9	58.2	58.2	1225-1232 bp	1.0	69.6
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCGTTGTTCATTC	50.0	45.0	58.2	56.7	1225-1232 bp	1.0	69.4
petN-psbM_p3	GGGACACGATTCACATGGAT	TGCTACTGCGTTGTTCATTCT	50.0	42.9	57.7	58.2	1246-1253 bp	1.0	68.1
petN-psbM_p4	GGGACACGATTCACATGGAT	TGCTACTGCGTTGTTCATTC	50.0	45.0	57.7	56.7	1246-1253 bp	1.0	68.0
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	TGCTACTGCGTTGTTCATTCT	50.0	42.9	57.6	58.2	1164-1171 bp	1.0	68.0