Hotspot

atpH-atpI

LSC · 15099-15763 · 665 bp

Back to Alseodaphne

Pi 0.00101

MarkerSeek score 59.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 665
variable_sites: 1
pi: 0.00101
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.0	57.9	750-752 bp	1.0	79.0
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCCGCAG	45.0	45.0	57.9	57.9	742-744 bp	1.0	78.8
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCCGCAG	50.0	45.0	57.9	57.9	806-808 bp	1.0	78.3
atpH-atpI_p4	GATACCTTCTACGGCTTGGC	TTTTGCAACTTTAGCCGCAG	55.0	45.0	58.1	57.9	835-837 bp	1.0	78.2
atpH-atpI_p5	CGATACCTTCTACGGCTTGG	TTTTGCAACTTTAGCCGCAG	55.0	45.0	57.9	57.9	836-838 bp	1.0	78.1