Hotspot

trnK-UUU-rps16

LSC · 4413-5232 · 820 bp

Back to Alseodaphne

Pi 0.00407
MarkerSeek score 50.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
820
variable_sites
5
pi
0.00407
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GAGGTATTTAAGGAATTTCGAAT 50.0 30.4 58.2 52.6 902-905 bp 1.0 40.8
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GAGGTATTTAAGGAATTTCGAAT 50.0 30.4 58.2 52.6 901-904 bp 1.0 40.8
trnK-UUU-rps16_p3 AAAAGACCATAGCCGCACTT GAGGTATTTAAGGAATTTCGAAT 45.0 30.4 57.8 52.6 923-926 bp 1.0 40.8
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA GAGGTATTTAAGGAATTTCGAAT 50.0 30.4 58.3 52.6 911-914 bp 1.0 40.8
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GAGGTATTTAAGGAATTTCGAATT 50.0 29.2 58.2 53.4 901-904 bp 1.0 40.8