Hotspot

rpl2-trnH-GUG

IRa · 159384-159426 · 43 bp

Back to Alpinia

Pi 0.0443

MarkerSeek score 70.20

Primer available yes

Label rpl2-trnH-GUG

Feature Metadata

label_name: rpl2-trnH-GUG
feature_type: igs
region: IRa
length_bp: 43
variable_sites: 6
pi: 0.0443
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnH-GUG_p1	GTTATCCTGCGCTTGGAAGA	TGGATCAAGGCAGTGGATTG	50.0	50.0	58.0	57.9	179-183 bp	1.0	85.4
rpl2-trnH-GUG_p2	GTTATCCTGCGCTTGGAAGA	GGATCAAGGCAGTGGATTGT	50.0	50.0	58.0	57.9	178-182 bp	1.0	85.4
rpl2-trnH-GUG_p3	GTTATCCTGCGCTTGGAAGA	GTGGATCAAGGCAGTGGATT	50.0	50.0	58.0	57.9	180-184 bp	1.0	85.4
rpl2-trnH-GUG_p4	GTTATCCTGCGCTTGGAAGA	CGGATGTAGCCAAGTGGATC	50.0	55.0	58.0	58.1	193-197 bp	1.0	85.2
rpl2-trnH-GUG_p5	GTTATCCTGCGCTTGGAAGA	TAGCTAGAAAAGGGGCGGAT	50.0	50.0	58.0	58.2	208-212 bp	1.0	84.5