Hotspot

psaC-ndhE

SSC · 122123-122816 · 694 bp

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Pi 0.029

MarkerSeek score 70.40

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 694
variable_sites: 53
pi: 0.029
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TTTGACCACAAAAGCCCGTA	TGCCGAAGCATCTATTGGAC	45.0	50.0	57.9	58.0	1089-1142 bp	1.0	87.5
psaC-ndhE_p2	ATAAGATGCAAGAGCCGACG	TGCCGAAGCATCTATTGGAC	50.0	50.0	58.1	58.0	1175-1228 bp	1.0	86.6
psaC-ndhE_p3	TTTGACCGCTGTACATTGCT	TGCCGAAGCATCTATTGGAC	45.0	50.0	58.1	58.0	1484-1537 bp	1.0	86.4
psaC-ndhE_p4	CTGGCCAAGCTGCTAAAGTA	TGCCGAAGCATCTATTGGAC	50.0	50.0	57.9	58.0	1427-1480 bp	0.25	53.8
psaC-ndhE_p5	TGGCCAAGCTGCTAAAGTAG	TGCCGAAGCATCTATTGGAC	50.0	50.0	57.9	58.0	1426-1479 bp	0.25	53.8