Hotspot

ccsA-ndhD

SSC · 119935-120246 · 312 bp

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Pi 0.0496

MarkerSeek score 75.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 312
variable_sites: 34
pi: 0.0496
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATATTGGAGTTGGGACCCCA	GGATTCTGGACCACGAGAAC	50.0	55.0	58.0	58.0	642-678 bp	1.0	89.1
ccsA-ndhD_p2	ATATTGGAGTTGGGACCCCA	TTGCATTGCCAGGAATGAGT	50.0	45.0	58.0	58.1	850-886 bp	1.0	86.8
ccsA-ndhD_p3	ACTGAAATGCACCAATCCGT	GGATTCTGGACCACGAGAAC	45.0	55.0	58.1	58.0	1043-1075 bp	1.0	86.7
ccsA-ndhD_p4	ATATTGGAGTTGGGACCCCA	CTATGCTGCTTCGACCTCTC	50.0	55.0	58.0	57.9	1140-1176 bp	1.0	85.0
ccsA-ndhD_p5	ATATTGGAGTTGGGACCCCA	CGGCCAACGGAATTTGAAAA	50.0	45.0	58.0	57.9	1119-1155 bp	1.0	85.0