Hotspot

ndhF-rpl32

SSC · 117097-117721 · 625 bp

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Pi 0.0312

MarkerSeek score 73.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 625
variable_sites: 58
pi: 0.0312
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TCGGTCGTGTGAACCAAAAT	TTGAACGTCCGGTAGAAACC	45.0	50.0	58.0	57.8	1254-1278 bp	1.0	84.0
ndhF-rpl32_p2	TACATGAAGGGGGAATTGCG	TTGAACGTCCGGTAGAAACC	50.0	50.0	57.9	57.8	1490-1499 bp	0.875	77.4
ndhF-rpl32_p3	GCGCAGATTTAGCAATTGCA	TTGAACGTCCGGTAGAAACC	45.0	50.0	58.1	57.8	1473-1482 bp	0.875	77.2
ndhF-rpl32_p4	TGCGCAGATTTAGCAATTGC	TTGAACGTCCGGTAGAAACC	45.0	50.0	58.1	57.8	1474-1483 bp	0.875	77.2
ndhF-rpl32_p5	ACGAGGAACTGACCCCATTA	TTGAACGTCCGGTAGAAACC	50.0	50.0	58.1	57.8	1419-1443 bp	0.75	72.8