Hotspot

psaJ-rpl33

LSC · 70265-70890 · 626 bp

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Pi 0.0224

MarkerSeek score 69.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 626
variable_sites: 38
pi: 0.0224
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GGATGCCTTGTCATTCCCAT	ACATCCTTACCCTTAGCCAT	50.0	45.0	57.9	55.6	635-716 bp	1.0	68.5
psaJ-rpl33_p2	ATCGTTTATTCCCGGATGCC	ACATCCTTACCCTTAGCCAT	50.0	45.0	58.1	55.6	648-729 bp	1.0	68.1
psaJ-rpl33_p3	TGGTTTGGGTCTTTAGCAGG	ACATCCTTACCCTTAGCCAT	50.0	45.0	57.7	55.6	685-766 bp	1.0	66.5
psaJ-rpl33_p4	CGTTTATTCCCGGATGCCTT	ACATCCTTACCCTTAGCCAT	50.0	45.0	58.3	55.6	646-727 bp	1.0	66.4
psaJ-rpl33_p5	TTATTCCCGGATGCCTTGTC	ACATCCTTACCCTTAGCCAT	50.0	45.0	57.7	55.6	643-724 bp	1.0	66.3