Hotspot

trnT-UGU-trnL-UAA

LSC · 48837-49489 · 653 bp

Back to Alpinia

Pi 0.0186

MarkerSeek score 69.30

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 653
variable_sites: 34
pi: 0.0186
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	55.0	50.0	57.9	58.0	703-1144 bp	1.0	84.8
trnT-UGU-trnL-UAA_p2	GAGTCGAACCGATGACCATC	ACCAATTTCGCCATATCCCC	55.0	50.0	58.2	58.0	734-1175 bp	1.0	84.4
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	711-1152 bp	1.0	84.2
trnT-UGU-trnL-UAA_p4	GAGTCGAACCGATGACCATC	TAGCGTCTACCAATTTCGCC	55.0	50.0	58.2	58.1	742-1183 bp	1.0	83.8
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	55.0	50.0	57.9	57.8	726-1167 bp	1.0	82.9