Hotspot

petN-psbM

LSC · 29662-30964 · 1303 bp

Back to Alpinia

Pi 0.0191

MarkerSeek score 68.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1303
variable_sites: 69
pi: 0.0191
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	940-1430 bp	1.0	76.1
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	939-1429 bp	1.0	76.1
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	998-1488 bp	1.0	75.6
petN-psbM_p4	ATGGGGAAGGAGTGGACTTT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	937-1427 bp	1.0	75.1
petN-psbM_p5	TGGGGAAGGAGTGGACTTTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	936-1426 bp	1.0	73.6