Hotspot

trnC-GCA-petN

LSC · 28723-29571 · 849 bp

Back to Alpinia

Pi 0.0166

MarkerSeek score 67.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 849
variable_sites: 42
pi: 0.0166
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	AGTTCAAATCTGGGTGTCGC	AGTCCCCCTAAAGTCCACTC	50.0	55.0	58.5	58.0	968-983 bp	1.0	81.5
trnC-GCA-petN_p2	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	898-913 bp	1.0	81.1
trnC-GCA-petN_p3	AGTTCAAATCTGGGTGTCGC	AGTCCACTCCTTCCCCATAC	50.0	55.0	58.5	58.1	957-972 bp	1.0	81.0
trnC-GCA-petN_p4	AGTTCAAATCTGGGTGTCGC	GTCCACTCCTTCCCCATACT	50.0	55.0	58.5	58.1	956-971 bp	1.0	81.0
trnC-GCA-petN_p5	GACATGGCCAAGTGGTAAGG	AGTCCCCCTAAAGTCCACTC	55.0	55.0	58.5	58.0	1014-1029 bp	1.0	80.7