Hotspot

ndhG-ndhI

SSC · 110838-111088 · 251 bp

Back to Alopecurus

Pi 0.0304
MarkerSeek score 66.10
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
251
variable_sites
18
pi
0.0304
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 TCTCATGTATTGGTCCAGGT TCGAATTCAAGAACGATTACTGA 45.0 34.8 55.5 56.1 303-319 bp 1.0 46.7
ndhG-ndhI_p2 TCTCATGTATTGGTCCAGGT TTCGAATTCAAGAACGATTACTGA 45.0 33.3 55.5 56.7 304-320 bp 1.0 46.7
ndhG-ndhI_p3 TCTCATGTATTGGTCCAGGT CTTCGAATTCAAGAACGATTACTGA 45.0 36.0 55.5 57.7 305-321 bp 1.0 46.7
ndhG-ndhI_p4 TCTCATGTATTGGTCCAGGT TCTTCGAATTCAAGAACGATTACTG 45.0 36.0 55.5 57.7 306-322 bp 1.0 46.7
ndhG-ndhI_p5 TCTCATGTATTGGTCCAGGT TCTTCGAATTCAAGAACGAT 45.0 35.0 55.5 52.7 306-322 bp 1.0 46.7