Hotspot

rpl32-trnL-UAG

SSC · 105440-106186 · 747 bp

Back to Alopecurus

Pi 0.0412

MarkerSeek score 67.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 747
variable_sites: 60
pi: 0.0412
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	846-918 bp	1.0	85.4
rpl32-trnL-UAG_p2	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	845-917 bp	1.0	85.4
rpl32-trnL-UAG_p3	AGGGTTTTTCTGGGCAACAA	ACTGAGATTGGAGAGCAGGT	45.0	50.0	58.1	58.1	1339-1411 bp	1.0	83.2
rpl32-trnL-UAG_p4	AAGGGTTTTTCTGGGCAACA	GATCCGCACAAAAGAGTTGC	45.0	50.0	58.1	58.0	1398-1470 bp	1.0	83.1
rpl32-trnL-UAG_p5	AGGGTTTTTCTGGGCAACAA	GATCCGCACAAAAGAGTTGC	45.0	50.0	58.1	58.0	1397-1469 bp	1.0	83.1