Hotspot

rpl22-rps19

LSC · 80414-80491 · 78 bp

Back to Alopecurus

Pi 0.059

MarkerSeek score 63.90

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 78
variable_sites: 10
pi: 0.059
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	AGATGGGATAAGACGCTCGA	CAAATTGGGGGAATTCGTGC	50.0	50.0	58.0	58.0	404 bp	1.0	82.8
rpl22-rps19_p2	AGATGGGATAAGACGCTCGA	GCAAATTGGGGGAATTCGTG	50.0	50.0	58.0	58.0	405 bp	1.0	82.8
rpl22-rps19_p3	GCCAGCGAATCTCATCAAGA	CAAATTGGGGGAATTCGTGC	50.0	50.0	58.1	58.0	341 bp	1.0	82.8
rpl22-rps19_p4	AGATGGGATAAGACGCTCGA	GGTTGGCCATACAATAGCGA	50.0	50.0	58.0	58.0	479 bp	1.0	82.1
rpl22-rps19_p5	AGATGGGATAAGACGCTCGA	GTTATCCTGCGCTTGGAAGA	50.0	50.0	58.0	58.0	946-949 bp	1.0	81.4