Hotspot

psbE-petL

LSC · 62234-63513 · 1280 bp

Back to Alopecurus

Pi 0.0121

MarkerSeek score 55.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1280
variable_sites: 30
pi: 0.0121
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GCCAATAAATAGAGCTGGGGT	45.0	47.6	58.0	57.8	1207-1476 bp	1.0	75.8
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCCAATAAATAGAGCTGGGGT	50.0	47.6	57.9	57.8	1196-1465 bp	1.0	75.6
psbE-petL_p3	ACCAACCCGCAATGAATAGG	GCCAATAAATAGAGCTGGGGT	50.0	47.6	58.2	57.8	1198-1467 bp	1.0	74.1
psbE-petL_p4	TGACAAATAACCAACCCGCA	AGCCAATAAATAGAGCTGGGG	45.0	47.6	58.0	57.5	1208-1477 bp	1.0	73.5
psbE-petL_p5	CAACCCGCAATGAATAGGGA	AGCCAATAAATAGAGCTGGGG	50.0	47.6	57.9	57.5	1197-1466 bp	1.0	73.3