Hotspot

trnF-GAA-ndhJ

LSC · 47370-47923 · 554 bp

Back to Alopecurus

Pi 0.0165

MarkerSeek score 56.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 554
variable_sites: 19
pi: 0.0165
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CGCGCCTTAAACGTATCCTA	50.0	50.0	58.1	57.9	681-698 bp	1.0	82.3
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	50.0	55.0	58.1	58.2	655-672 bp	1.0	81.6
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATGATAATCATCCGCGCCTT	50.0	45.0	58.1	57.5	693-710 bp	1.0	79.4
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGGTGGC	50.0	50.0	58.1	57.5	656-673 bp	1.0	79.4
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCCGCGCCTTAAACGTATC	50.0	50.0	58.1	58.5	684-701 bp	1.0	79.3