Hotspot

trnT-UGU-trnL-UAA

LSC · 45551-46379 · 829 bp

Back to Alopecurus

Pi 0.0139

MarkerSeek score 59.30

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 829
variable_sites: 25
pi: 0.0139
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.0	58.0	915-943 bp	1.0	83.2
trnT-UGU-trnL-UAA_p2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.1	58.0	907-935 bp	1.0	82.9
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	55.0	50.0	57.9	58.0	885-913 bp	1.0	82.5
trnT-UGU-trnL-UAA_p4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.0	58.2	905-933 bp	1.0	81.3
trnT-UGU-trnL-UAA_p5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	50.0	50.0	58.0	57.7	914-942 bp	1.0	81.0