Hotspot

trnC-GCA-rpoB

LSC · 18210-19385 · 1176 bp

Back to Alopecurus

Pi 0.0179

MarkerSeek score 52.80

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1176
variable_sites: 44
pi: 0.0179
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	ATTCCCTCGTTTCCATTCCG	50.0	50.0	58.1	58.0	1238-1250 bp	1.0	83.2
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	50.0	50.0	58.1	57.8	1245-1257 bp	1.0	82.1
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1253-1265 bp	1.0	80.9
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1252-1264 bp	1.0	80.9
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	GGACATTCCCTCGTTTCCAT	50.0	50.0	58.1	57.6	1242-1254 bp	1.0	80.2