Hotspot

infA

LSC · 78889-79071 · 183 bp

Pi 0.0255

MarkerSeek score 55.40

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 183
variable_sites: 7
pi: 0.0255
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	ATAACTTCCGCTCGTTGCAT	ACGGTTCGAATAGCATCCAC	45.0	50.0	58.0	58.1	1302-1311 bp	1.0	81.0
infA_p2	GAAACCACTCGACCTCGTAC	ACGGTTCGAATAGCATCCAC	55.0	50.0	58.0	58.1	1176-1185 bp	1.0	81.0
infA_p3	TCAGTCGGCACTTTTCACAA	ACGGTTCGAATAGCATCCAC	45.0	50.0	58.0	58.1	827-837 bp	1.0	80.9
infA_p4	CCCGTAATGCTGCATCTCTT	ACGGTTCGAATAGCATCCAC	50.0	50.0	58.0	58.1	1343-1352 bp	1.0	80.9
infA_p5	AATGCTGCATCTCTTCCGAG	ACGGTTCGAATAGCATCCAC	50.0	50.0	58.0	58.1	1338-1347 bp	1.0	80.8