Hotspot

rpl20-clpP1

LSC · 67877-68873 · 997 bp

Back to Aloe

Pi 0.0127
MarkerSeek score 62.90
Primer available yes
Label rpl20-clpP1

Feature Metadata

label_name
rpl20-clpP1
feature_type
igs
region
LSC
length_bp
997
variable_sites
18
pi
0.0127
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-clpP1_p1 TCCTCGTCTAACTCTGGTCA CCAAGCTCATGGCATTGTTG 50.0 50.0 57.1 58.0 1082-1118 bp 1.0 74.4
rpl20-clpP1_p2 TCCTCGTCTAACTCTGGTCA TATGTCAGCAACAGAAGCCC 50.0 50.0 57.1 57.9 1100-1136 bp 1.0 73.5
rpl20-clpP1_p3 GCCCCTATAAAGGTTGATGCA CCAAGCTCATGGCATTGTTG 47.6 50.0 58.1 58.0 1141-1177 bp 1.0 72.9
rpl20-clpP1_p4 GCCCCTATAAAGGTTGATGCA TATGTCAGCAACAGAAGCCC 47.6 50.0 58.1 57.9 1159-1195 bp 1.0 72.0
rpl20-clpP1_p5 TCCTCGTCTAACTCTGGTCA TCCGAAGACATGGAAAGGGA 50.0 50.0 57.1 58.3 1126-1162 bp 1.0 71.6