Hotspot

trnS-GCU-trnG-UCC

LSC · 8211-8695 · 485 bp

Back to Aloe

Pi 0.0266
MarkerSeek score 62.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
485
variable_sites
19
pi
0.0266
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 AGATTAGCAATCTGCCGCTT GGGAAAAAGGACGAAACCCT 45.0 50.0 57.7 58.0 647-663 bp 1.0 81.6
trnS-GCU-trnG-UCC_p2 CGGAGAGAGAGGGATTCGAA GGGAAAAAGGACGAAACCCT 55.0 50.0 58.3 58.0 693-709 bp 1.0 81.5
trnS-GCU-trnG-UCC_p3 TTCCTCGTTCTGACCTTCCA GGGAAAAAGGACGAAACCCT 50.0 50.0 58.3 58.0 1169-1189 bp 1.0 80.8
trnS-GCU-trnG-UCC_p4 TTCCTCGTTCTGACCTTCCA CATCGTTAGCTTGGAAGGCT 50.0 50.0 58.3 58.0 1769-1792 bp 1.0 80.2
trnS-GCU-trnG-UCC_p5 TTCCTCGTTCTGACCTTCCA CGTTAGCTTGGAAGGCTAGG 50.0 55.0 58.3 58.1 1766-1789 bp 1.0 80.0