Hotspot

trnN-GUU-ndhF

IRb · 116186-117389 · 1204 bp

Back to Alocasia

Pi 0.358

MarkerSeek score 80.00

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1204
variable_sites: 346
pi: 0.358
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	CGGGAGAATTGATGACTGCA	TGGTTGGCTCCATCGAAAAA	50.0	45.0	58.0	58.0	2056-2143 bp	1.0	91.7
trnN-GUU-ndhF_p2	GCCAGGTATTGACGGTGATT	TGGTTGGCTCCATCGAAAAA	50.0	45.0	58.0	58.0	1941-2028 bp	1.0	91.6
trnN-GUU-ndhF_p3	CGGATCTACGCCAGGTATTG	TGGTTGGCTCCATCGAAAAA	55.0	45.0	57.9	58.0	1950-2037 bp	1.0	91.4
trnN-GUU-ndhF_p4	ATTATCCCGGATCTACGCCA	TGGTTGGCTCCATCGAAAAA	50.0	45.0	58.1	58.0	1957-2044 bp	1.0	91.4
trnN-GUU-ndhF_p5	CGGGAGAATTGATGACTGCA	AGTTTCTTTGCGGGTGAACT	50.0	45.0	58.0	57.9	1684 bp	0.5	59.4