Hotspot

petA-psbJ

LSC · 68152-69281 · 1130 bp

Back to Alocasia

Pi 0.0784
MarkerSeek score 85.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1130
variable_sites
83
pi
0.0784
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 AGCACAAATCTTTTTGGTTCT TCCGGATTAGGCTCATCTCT 33.3 50.0 54.5 57.3 1193-1224 bp 1.0 49.5
petA-psbJ_p2 AGCACAAATCTTTTTGGTTCT CCGGATTAGGCTCATCTCTG 33.3 55.0 54.5 57.3 1192-1223 bp 1.0 49.5
petA-psbJ_p3 AGCACAAATCTTTTTGGTTCTT TCCGGATTAGGCTCATCTCT 31.8 50.0 55.3 57.3 1193-1224 bp 1.0 49.5
petA-psbJ_p4 AGCACAAATCTTTTTGGTTCTT CCGGATTAGGCTCATCTCTG 31.8 55.0 55.3 57.3 1192-1223 bp 1.0 49.5
petA-psbJ_p5 AGCACAAATCTTTTTGGTTCT TTCCGGATTAGGCTCATCTCT 33.3 47.6 54.5 58.0 1194-1225 bp 1.0 49.5