Hotspot

accD-psaI

LSC · 63605-64206 · 602 bp

Back to Alocasia

Pi 0.0935

MarkerSeek score 80.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 602
variable_sites: 55
pi: 0.0935
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCACAAGCGGCTGAGTATTC	GGAGTCACATGTCGTACCAC	50.0	55.0	58.0	58.0	936-1652 bp	1.0	84.8
accD-psaI_p2	TCACAAGCGGCTGAGTATTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	792-1509 bp	1.0	84.8
accD-psaI_p3	TCACAAGCGGCTGAGTATTC	CCGCTTTTGACCAGAGCTAT	50.0	50.0	58.0	58.0	1033-1749 bp	1.0	84.5
accD-psaI_p4	AGTCGGCGAGAAAATAACCC	GGAGTCACATGTCGTACCAC	50.0	55.0	58.0	58.0	1261 bp	0.5	59.8
accD-psaI_p5	AGTCGGCGAGAAAATAACCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1117 bp	0.5	59.6