Hotspot

trnF-GAA-ndhJ

LSC · 51759-53113 · 1355 bp

Back to Alocasia

Pi 0.104

MarkerSeek score 77.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 1355
variable_sites: 86
pi: 0.104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.61
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	944-1465 bp	1.0	88.3
trnF-GAA-ndhJ_p2	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	987-1508 bp	1.0	86.8
trnF-GAA-ndhJ_p3	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	986-1507 bp	1.0	86.8
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CGCGCCTTAAACGTATCTTG	50.0	50.0	58.1	57.7	969-1490 bp	1.0	86.4
trnF-GAA-ndhJ_p5	TGGAAAATGGTCGGGATAGC	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.7	57.9	992-1513 bp	1.0	85.7