Hotspot

trnS-GCU-trnS-CGA

LSC · 8820-10649 · 1830 bp

Back to Alocasia

Pi 0.147

MarkerSeek score 80.80

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 1830
variable_sites: 216
pi: 0.147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	GCTTTAGTCCACTCAGCCAT	AGGACGAATCACACTTTTACCA	50.0	40.9	57.6	57.7	1776-1893 bp	1.0	70.0
trnS-GCU-trnS-CGA_p2	GCTTTAGTCCACTCAGCCAT	GGACGAATCACACTTTTACCAC	50.0	45.5	57.6	57.6	1775-1892 bp	1.0	69.5
trnS-GCU-trnS-CGA_p3	AGTCCACTCAGCCATCTCTC	AGGACGAATCACACTTTTACCA	55.0	40.9	58.5	57.7	1771-1888 bp	1.0	69.1
trnS-GCU-trnS-CGA_p4	AGTCCACTCAGCCATCTCTC	GGACGAATCACACTTTTACCAC	55.0	45.5	58.5	57.6	1770-1887 bp	1.0	68.7
trnS-GCU-trnS-CGA_p5	TAGTCCACTCAGCCATCTCT	AGGACGAATCACACTTTTACCA	50.0	40.9	57.2	57.7	1772-1889 bp	1.0	66.5